I have designed an siRNA targeting a specific gene using OligoWalk software. The selected siRNA does not form hairpins at 37°C and binds effectively to the coding sequence (CDS) of the target mRNA. The binding is confirmed in the two-dimensional structure, where it attaches to the single-stranded 5' nucleotides of the mRNA and predominantly to the single-stranded region of the 3' nucleotides. The GC content of the siRNA is approximately 31%, with the 5' end starting with a U and the 3' end with a G. Additionally, the seed region contains more than 4 A/U nucleotides. The siRNA sequence is 19 nucleotides long, extended to 21 nucleotides by adding 2 TTs to the 3' end.

Given that I could not identify an siRNA with all these characteristics using other software, and considering that OligoWalk has not been updated since 2008, I am seeking feedback on the potential efficiency and functionality of the siRNA I designed. Is it likely to be effective?