I am reinvigorating L.major promastigote cell lines using m-199 medium supplemented with 10% FBS and to avoid any sort of bacterial contamination, i am using pen strep and what should be the optimum dose of this antibiotics because i was wondering if higher dose has an inhibition effect on revival rate as it has on other strains such as L.tropica. Please share your wisdom.
Deepak K. Goyal
Hi Mebrahtu,
1. In my opinion you have to standardized it.
2. You can try 20/30/40 ug/mL concentration.
3. Observe 2-3 concentration for better results.
Thank you,
Good luck
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