Side effects of COVID-19 Vaccination?

It is good milestone to start vaccinating against COVD-19. Are there any unusual side effects and strange signs during and after vaccination?

14 December 2020 1,005 5 View

Dear sir/madam could you help me in how to determine the sample size and use of the 50% proportion for sample size estimation?

I propose to determine factors influencing bacteriological and clinical cure in dairy cows with clinical mastitis after treatment with antibiotics. I propose to follow clinically healthy (i.e....

19 February 2020 5,505 2 View

Could anyone help in explaining the origin of such a dense region of ferrite at weld-interface of steel rods during resistance upset butt welding?

-Steel grade of 20MnB4 - Resistance upset butt welding of chains

07 June 2017 632 7 View

Could anyone guide me in performing fully coupled thermal-electrical-structural analysis in Abaqus, for simulating upset resistance butt welding?

05 June 2017 6,459 2 View

No title

Formula used for sample size calculation for a survey: n=z2 *p(1-p)/d2 or with corrected factor. Likelihood of having controlled blood glucose level: (OR 95% CI 1.50 [1.23;1.76]) in result section...

12 January 2016 3,169 2 View

Any suggestions for drought resistant rootstock for apple?

Read 3 answers by scientists to the question asked by Getachew Berhe on Mar 17, 2010

16 March 2010 6,975 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View