Does anyone know the optimal concentration and volume of dsRNA to inject nymph ticks, for RNA interference downstream study ?

More Mebrahtu B. Gebremedhin's questions See All

How can we select the reference model for a nonlinear dynamic of 2 dof robot?

in designing and simulation of a nonlinear dynamics of 2 dof robotic manipulator using model reference adaptive control system, how can we select the reference model to the robot? or what can we...

05 December 2023 4,529 1 View

Relationship of burnout with knowledge and skill?

Assume I have collected a data on burnout, knowledge and skill from health workers in a given time and place. so can I do an analysis on the relationship of burnout with knowledge and skill of...

05 June 2022 7,056 1 View

What data analysis method(s) could be applied under purposeful sampling?

I have conducted a survey using purposeful sampling and now I need to proceed with data analysis. However, I could not find adequate information on the best way of analyzing data collected using...

09 June 2021 2,201 3 View

Could CBR value obtained using DCP field test (UK DCP Software 3.1) of unbound materials of flexible pavements is more than 100%?

Why the CBR value obtained using DCP field test (UK DCP Software 3.1) of unbound materials of flexible pavements is more than 100%. Does there are conditions CBR can also be more than 100%. The...

17 March 2021 348 0 View

Could any one help me with ABAQUS tutorials on fiber reinforced polymer concrete columns please?

I am doing my thesis research in structural engineering, on a title Glass Fiber Reinforced Polymer confined concrete column.

24 December 2020 9,293 0 View

Recombinant protein purification, fail to elute the GST tagged protein using a reduced glutathione buffer?

My GST tagged, recombinant ，target protein of 10 kDa， expressed in BL21, was failed to be eluted in a 16mM of reduced glutathione buffer。However， I find my protein down，binded with the GST bind...

18 October 2020 7,226 3 View

How to use Geographical Weighted Regression (GWR) in ArcGIS?

How to iterate coefficient and residual maps for daily time series rainfall with elevation as explanatory variable in ArcGIS ?

06 February 2019 4,163 3 View

Effect of high dose Pen Strep on promastigote revival?

I am reinvigorating L.major promastigote cell lines using m-199 medium supplemented with 10% FBS and to avoid any sort of bacterial contamination, i am using pen strep and what should be the...

15 August 2017 6,028 1 View

Looking for proper data analysis?

I have 20 independent variables regarding the practices and attitude of fishers about fisheries management. Data was collected though questionnaire and recorded as YES/NO. I would like to identify...

09 August 2017 9,432 8 View

Is there anyone who can give me some explanation about two way classification chi square?

To see if fish species distribution is dependent on area, I have collected fish sample for one year from four selected sampling site of a given lake. Thus, ten fish species were recorded from all...

05 May 2017 5,234 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View