I am trying to test the effect of Osmolytes on protein folding using circular dichroism. Osmolytes, such sucrose, TMAO and Glycine Betaine are used. I found big background noise when using Glycine betaine within normal wavelength range (200nm-250nm). I used 50mM phosphate buffer at pH 7.0. All other osmolytes show low noise close to 0.
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pubmed/22987585
Thanks
Corroborating Achim and in accordance with your results, glycine betaine (GB) interferes in the Far UV CD region. In reference to the citation by Isam, the paper in the suggested link (Kishore and co-workers) confirms that GB could not be used in Far UV CD measurements. However, the authors have used the Near UV CD range (i.e.,~ 260-300 nm) to study the effect of this osmolyte on protein folding. The caveat is that the Near UV CD requires larger protein concentrations (typically >1 mg/ml) and larger volumes (1 cm pathlength cell).
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