Xinji Zhu

6 Questions 6 Answers 0 Followers

Questions related from Xinji Zhu

Protein sequence by Mass Spec, the coverage is not 100%?

Hi, the coverage for my protein sample after Mass Spec is around 95%. Is there any reason why the coverage is not 100%? Sample purity? some peptides not identified by mass spec? Thank you!

12 March 2020 9,402 0 View

Differential Scanning Calorimetry (DSC) protein aggregation?

I am working on Differential Scanning Calorimetry (DSC) for my protein. Mainly I want to check its melting temperature and enthalpy changes. I first tried two standard proteins ( BSA and...

18 February 2020 1,823 3 View

Polydispersity Index(PDI) in dynamic light scattering(DLS) ?

Hi, I am using DLS to test the size change of my protein in different conditions. the Z-average is around 20nm with PDI around 0.2. I was told that the PDI is too high and the reasonable PDI...

10 July 2019 1,180 11 View

Effect of Glycine betaine on circular dichroism (CD) spectra of protein ?

I am trying to test the effect of Osmolytes on protein folding using circular dichroism. Osmolytes, such sucrose, TMAO and Glycine Betaine are used. I found big background noise when using...

06 June 2019 8,388 4 View

Circular dichroism (CD) value at 222nm and 208nm issue

Hi, I am currently analyzing a thermal denaturation of protein by CD, from the spectra, there are two peaks at around 222nm and 208nm. I know that for a helix, it should have a negative value near...

01 January 1970 3,905 6 View

Can Halophilic protein be considered as Intrinsic disordered proteins(IDPs) in the absence of salt

a lot of halophilic protein is unfolded in the absence of high concentration of salt where most proteins favors the folded state. I wonder if such halophilic protein can be considered as IDPs in...

01 January 1970 8,422 3 View