14 Questions 9 Answers 0 Followers
Questions related from Xinji Zhu
I found that the solution is light yellow. Is that normal?
24 September 2024 4,990 0 View
I am trying to get a protein complex (one is around 110kDa and the other is around 25kDa) for Cryogenic electron microscopy (cryo-EM). The main method I am using is to run the sample through a gel...
29 August 2023 6,839 1 View
I got two ΔG, one is from the equilibrium constant( ΔG=-RTlnK), the equilibrium constant(K) is from protein kinetic experiments (K=Kfold/Kunfold at H20). Another ΔG is from equilibrium folding...
01 May 2020 6,655 4 View
Hi, the coverage for my protein sample after Mass Spec is around 95%. Is there any reason why the coverage is not 100%? Sample purity? some peptides not identified by mass spec? Thank you!
12 March 2020 9,444 0 View
I am trying to get enthalpy change for thermal unfolding of my protein using differential scanning calorimetry(DSC). The graph was attached below. Instead of two parallel baselines in normal DSC...
23 February 2020 4,942 4 View
I am working on Differential Scanning Calorimetry (DSC) for my protein. Mainly I want to check its melting temperature and enthalpy changes. I first tried two standard proteins ( BSA and...
18 February 2020 1,943 3 View
Hi, I am working on the protein denaturation by circular dichroism. I have tried both GdnHCl and urea and it did not work well. I want to try stronger denaturants, such as GdnSCN and Thiourea....
13 February 2020 8,546 3 View
Hi, I am using DLS to test the size change of my protein in different conditions. the Z-average is around 20nm with PDI around 0.2. I was told that the PDI is too high and the reasonable PDI...
10 July 2019 1,219 11 View
I am trying to test the effect of Osmolytes on protein folding using circular dichroism. Osmolytes, such sucrose, TMAO and Glycine Betaine are used. I found big background noise when using...
06 June 2019 8,470 4 View
I want to compare the difference in amino acid composition among psychrophile, mesophile and thermophile. Is there any way to get the amino acid composition for the whole proteome ? Thanks！
27 March 2019 1,693 4 View
Hi, I am currently using Gdnhcl to analyze folding of my protein. I found the protein reach equilibrium at transition region(1.4M Gdnhcl) very fast(around 3mins). So I decide to use titration...
14 November 2018 890 2 View
I am starting to design my primers for cloning. In one protocol, it says that avoid a T as ultimate base at the 3' end. I wonder what will happen if I use T as ultimate base at the 3' end. Is it a...
09 November 2015 2,504 1 View
Hi, I am currently analyzing a thermal denaturation of protein by CD, from the spectra, there are two peaks at around 222nm and 208nm. I know that for a helix, it should have a negative value near...
01 January 1970 3,949 6 View
a lot of halophilic protein is unfolded in the absence of high concentration of salt where most proteins favors the folded state. I wonder if such halophilic protein can be considered as IDPs in...
01 January 1970 8,486 3 View
