Which model is the simplest for the induction of Epithelial to Mesenchymal transition and in which cell line? I have tried several cell lines like MCF-7 and although I do get an up-regulation in vimentin with twist transfection I don't get changes in cell morphology and only very minor changes in cell surface markers like CD44.
Anything stronger and more dramatic?
We have investigated this question further. We found TGF-beta induced EMT in MCF-12A as one of the best models for studying EMT at least for our story. Cancer cell lines are less sensitive to EMT induction. Our results are now published:
Alsuliman el at. Bidirectional crosstalk between PD-L1 expression and epithelial to mesenchymal transition: Significance in claudin-low breast cancer cells. Molecular Cancer 2015, 14:149
R&D Systems offers StemXVivo EMT Inducing Media Supplement (100X) [Catalog# CCM017] which is useful to induce EMT in vitro in less than a week (5 days). This medium contains a mixture of EMT-inducing recombinant proteins (Wnt-5a and TGF-beta1) and neutralizing antibodies (anti- E-Cadherin, anti- sFRP-1 and anti-Dkk-1) for induction of EMT. Since this product has a defined composition, one can expect consistent results with little or no experiment to experiment variation. This medium is compatible with several different cell lines such as MCF-7, MCF-10A, HT-29, A549 and A431.
For ~20 different frequently asked questions and troubleshooting tips on EMT & Stemness, I would also suggest checking out this Novus' resource: EMT & Stemness FAQs http://www.novusbio.com/support/emt-and-stemness-faqs
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