Graphpad prism software full version?

Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...

03 April 2018 4,492 12 View

Is there any difference between shannon weiner index and simpson index?

What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...

02 March 2018 1,778 2 View

Dgge sequence analysis using SNAPGENE?

I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...

01 February 2018 8,731 5 View

LPS assay to determine endotoxemia in mice model?

If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest . Thanks in advance

31 December 2017 2,264 0 View

Skimmed milk or BSA blocking?

Which blocking agent gives more clearer result for Internal control antibody like beta actin ?

31 December 2017 8,520 0 View

Is it necessary to isolate and amplify clones before Sanger sequencing?

Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards

10 November 2017 7,534 8 View

Analysis of Intestinal Permeability in mice through FITC-dextran method?

Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...

10 November 2017 8,319 7 View

Physico chemical characterization of Polysaccharide?

What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks

10 November 2017 8,919 3 View

Structure of Polysaccharide?

I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...

10 November 2017 9,598 6 View

How to get the best DGGE gel image?

How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.

10 November 2017 9,570 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View