How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.
Hello Dr Sadia
Please follow the following RG links
https://www.researchgate.net/post/Good_DGGE_image_for_publication
https://www.researchgate.net/post/Picture_is_attached_What_can_be_done_to_produce_a_DGGE_gel_with_good_quality
https://www.researchgate.net/post/Low_intensity_of_bands_in_DGGE
https://www.researchgate.net/post/Can_anyone_suggest_best_way_to_image_DGGE_gels_Is_the_percentage_of_gel_important1
https://www.researchgate.net/post/Why_do_my_DGGE_gels_not_spread_very_far_apart_have_difficutly_spreading_out_and_down_the_gel
Hope this will help you
Cheers.....
Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...
03 April 2018 4,675 12 View
What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...
02 March 2018 2,047 2 View
I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...
01 February 2018 8,883 5 View
If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest . Thanks in advance
31 December 2017 2,392 0 View
Which blocking agent gives more clearer result for Internal control antibody like beta actin ?
31 December 2017 8,645 0 View
Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards
10 November 2017 7,715 8 View
Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...
10 November 2017 8,473 7 View
What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks
10 November 2017 9,127 3 View
I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...
10 November 2017 9,736 6 View
Brightness or contrast of an image can be adjusted by using PS for scientific publications? Is it legal to adjust using this software for publication ?
09 October 2017 2,031 7 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View