If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest .
Thanks in advance
Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...
03 April 2018 4,675 12 View
What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...
02 March 2018 2,047 2 View
I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...
01 February 2018 8,883 5 View
Which blocking agent gives more clearer result for Internal control antibody like beta actin ?
31 December 2017 8,645 0 View
Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards
10 November 2017 7,715 8 View
Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...
10 November 2017 8,473 7 View
What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks
10 November 2017 9,127 3 View
I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...
10 November 2017 9,736 6 View
How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.
10 November 2017 9,702 1 View
Brightness or contrast of an image can be adjusted by using PS for scientific publications? Is it legal to adjust using this software for publication ?
09 October 2017 2,031 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Difficulty with permittivitt and Magnetic Permeability Calculations Hello everyone, I have all the parameters related to the calculations of the permittivitty and magnetic permeability...
30 July 2024 5,206 1 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View