Greetings,
I'm trying to stimulate isolated primary B cells using CpG ODN and
DOTAP mixture. However, because I've never used DOTAP, it seems I'm having a difficulty to make a proper DOTAP + ODN mixture. I used Roche DOTAP (400uL) and CpG ODN. I dissolved CpG ODN in endotoxin free water, and mixed with DOTAP in 1:3 ratio (DNA 1ug : DOTAP 3uL).
Any help would be greatly appreciated.
Thanks a lot.