Greetings,

I'm trying to stimulate isolated primary B cells using CpG ODN and

DOTAP mixture. However, because I've never used DOTAP, it seems I'm having a difficulty to make a proper DOTAP + ODN mixture. I used Roche DOTAP (400uL) and CpG ODN. I dissolved CpG ODN in endotoxin free water, and mixed with DOTAP in 1:3 ratio (DNA 1ug : DOTAP 3uL).

Any help would be greatly appreciated.

Thanks a lot.

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