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Questions related from Joo Sung Gabriel Shim
Hi guys! I'm trying to perform NP-KLH injection for the first time. It seems many protocols recommend using the mixture of NP-KLH with alum or Freud's adjuvant. Due to my inexperience in this...
02 September 2020 2,811 0 View
Hello all, I'm just wondering whether I can use Mitotracker green (MTG) for microplate experiment (96 well, fluoresence reader). Any help would be greatly appreciated! Thanks!
06 January 2020 403 1 View
Hi all, I'm just wondering the difference between NP-KLH and NP-CGG for antigen specific B cell study in germinal center. Any help would be greatly appreciated. Thanks!
21 December 2019 784 4 View
Greetings, I'm trying to stimulate isolated primary B cells using CpG ODN and DOTAP mixture. However, because I've never used DOTAP, it seems I'm having a difficulty to make a proper DOTAP +...
20 March 2018 9,256 2 View
Dear colleagues, I'm trying to isolate B cells from mouse spleen and subsequently culture them about 24 hours. My question is, do I need stimulants (eg. IL-4, CD40L) other than basic culture...
06 February 2018 895 4 View
