Best way to draw COLONY output / pedigrees?

Hi, I am using the sibship analysis software COLONY 2.0.6.6 (most recent version) on about 150 individuals offspring, with some of them as potential fathers and mothers. COLONY provides a lot...

02 February 2021 6,910 2 View

Format Mothur database to run through dada2?

I am looking to run illumina miseq data through dada2, but the limited identification of this gene means there are no pre-build databases. I have a database built in MOTHUR format, does anyone...

30 November 2020 8,228 3 View

How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

Has anyone used paraffin oil (liquid paraffin) instead of saturated alkane mixture in determining RI?

I would like to know if anyone has tried, or has any experience, in analyzing liquid paraffin with GC/MS and recording retention times. As hypothesized, this alkane mixture could maybe serve as a...

14 November 2019 8,547 3 View

Can I construct and ARDL model and test for cointegration with i(1), i(0) and i(2) variables?

Hi, I am currently doing a dissertation on demographic determinants of household savings in China during the One Child Policy, using time series. My variables are as follows: Household Savings- %...

21 July 2019 9,085 21 View

Rotating airfoil simulation - Fluent ansys - CL, CD?

Hello guys, I am in real need, I am working on my diploma thesis and the deadline is really close. I am having a bit of trouble with getting correct results of CL and CD from simulation over a...

13 April 2019 6,666 7 View

How do I increase the quality of the resulted video from Ansys Fluent simulation?

Hey! I am working on a flow analysis over a 2D airfoil.. I simulated the situation at different angles of attack, put it at 400 interations and everything works well in programme, although after...

09 April 2019 8,878 3 View

Is it possible to extract lift and drag curve from Ansys fluent?

Hey! I am currently working on flow analysis over 2D airfoil in Fluent ansys... I am new in this programme so after a day of hard work I managed to make the simulation according to the youtube...

09 April 2019 519 3 View

What cell culture contaminant turns media yellow but not turbid?

20 May 2015 4,389 11 View

Have anyone experienced that A549 and U251 cells grow in regular wavy lines in T75 flasks?

I have occasionally experienced that the two cell lines have grown in very regular wavy lines from the stopper to the opposite end of the flask. The cells seems to be okay except that they won't...

15 April 2015 9,034 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View