I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no 1) and another time I worked in a late passage (such as no.4). Does the number of passages show a type of resistant or not to the cytotoxic drugs? The shape and the identity of a cell still conserved. How many passages after it should I withdraw the cell? The cell line is MDA-MB-231.
Sudip Mukherjee
SURELY, always better to use the cells of initial passages. You should carefully see the size and shape of your cell, any alteration you should reject it.
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Sudip Mukherjee
It depends, carefully see any microplasma contamination is there or not?
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