Khatereh Khorsandi

10 Questions 17 Answers 0 Followers

Questions related from Khatereh Khorsandi

Dose anyone has experience with RNA isolation from isolated keratinocytes cells from wound edge?

My question is how I can increase the quantity of RNA isolated from cells in wound edge in this case from keratinocytes? I have done the experiment and still the quantity of isolated RNA is low...

12 April 2023 2,042 4 View

Does anyone know antibodies that are common for flow cytometry of keratinocyte? specially for their sorting ...?

06 September 2022 2,820 0 View

Does anyone know how can I use vitamin D in cell culture?

As you know vitamin D is not water soluble, I need use it in cell culture media that is watery , can you help me?

14 November 2015 5,341 6 View

Does anyone know that aminolevulinic acid (ALA) has absorbance in water solution (in vitro) by UV/Vis spectroscopy or not? why?

aminolevulinic acid (ALA), PDT, Drug

12 June 2015 4,414 3 View

Can we dye cells with alizarin red S or oil red with out fixing cells?

dye cells with alizarin red S or oil red( for differentiation test)

21 February 2015 3,480 1 View

How to measure total ROS measurement ?

Is there any protocol to measure intracellular ROS using spectrophotometric method?

09 February 2015 6,452 6 View

Is there a dilution factor for trypan blue exclusion assay, when we use 90 micromolar cell sample and 10 micromolar trypan blue?

Trypan blue exclusion assay dilution factor

03 December 2014 5,685 2 View

Does the number of cell passages affect the activity of drug or metal?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no...

01 December 2014 6,671 4 View

What is the best method for detecting singlet oxygen in cell culture studies?

Do you have any suggestion on assays for detecting singlet oxygen in cell culture?

28 October 2014 1,463 1 View

Doxorubicin hydrochloride: Has doxorubicin in own molecule net positive charge or not?

Hello I have a question about doxorubicin hydrochloride ? has doxorubicin in own molecule net positive charge or not? I mean when we dissolve doxorubicin hydrochloride in distilled water...

01 January 1970 4,102 9 View