Does anyone know antibodies that are common for flow cytometry of keratinocyte? specially for their sorting ...?

06 September 2022 0 3K Report

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Science method

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What are the definitions for Annual moringa and Perennial moringa?

The fantastic tree Moringa oleifera is a perennial tree. In literature, I see moringa being referred to as Annual moringa and Perennial moringa. What are the basis for these terminology? Is there...

09 December 2023 8,364 0 View

Hydrochar production at farm scale by farmers?

Large quantities of agricultural wastes are produced in farms. Is there any farmer-friendly, practical and small scale hydrothermal carbonization (HTC) units for production of hydrochar, to...

24 July 2023 6,505 5 View

Best methods for organic paddy-rice production?

Is organic paddy rice production at commercial scale popular? I greatly appreciate some excellent, practical references on this subject. Thank you

24 July 2023 3,923 0 View

Dose anyone has experience with RNA isolation from isolated keratinocytes cells from wound edge?

My question is how I can increase the quantity of RNA isolated from cells in wound edge in this case from keratinocytes? I have done the experiment and still the quantity of isolated RNA is low...

11 April 2023 1,995 4 View

Journals on Indiginous knowledge in agriculture or traditional agricultural systems?

Greetings I am intending to publish some papers on historical, indigenous and traditional production of agricultural crops. I would greatly appreciate anyone who could introduce some respected and...

15 May 2022 1,504 0 View

If it possible we detect and distinguish LOS path from NLOS path in received signals with deep learning or machine learning approaches?

I want to estimate AOA and TOA of LOS path from superposition signal that received in receiver.

20 May 2021 355 6 View

What are the best and easiest to handle SRB species for sulfate reduction studies?

I have a sample of fat and I want to reduce sulfate in it without breaking the fats, what are the best SRB species to use? I have found Desulfovibrio vulgaris to be the model microorganism in many...

04 April 2021 8,938 8 View

Can Graphene be produced from Biochar?

An interesting and very useful material now a days is Graphene. Has there been any research on manufacturing Graphene from biochar? If so, any references please? Thank you

24 August 2020 6,670 5 View

Can we produce electricity from salt waters (for example saline ground waters or salty rivers), efficiently?

Can we produce electricity from salt waters (for example saline ground waters or salty rivers), efficiently? Could you plz give some references or elaborate on that. Thank you

24 March 2020 9,339 15 View

When the biochar is ready in a lab scale pyrolyzer?

When making biochar for research purposes in a lab or workshop, using systems like muffle furnace or fixed bed pyrolyser or other pyrolyser systems, how do we know then when the biochar is ready...

08 March 2019 4,108 8 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View