Dear all,
I am working on identification of exosome related protein markers through western blot antibody probing. In my samples, there is an impurity seen at 66KDa which is obtained during the sample acquisition process. When I am screening for the exosomal marker proteins, the impurity is also binding with the antibody and another unwanted constant band is also seen at35KDa during blot imaging. Here I have used Biorad Precision dual color ladder as a protein standard
I am unable to sort this problem. Help me If any one could.
Hi...I think your source of exosome isolation might be from plasma/serum samples...? if so that prominent huge band you are seeing in your blot should be any kind of serum/plasma proteins..but i dont think your westerns can be affected with this,since we are using target specific antibodies....
Exosome marker antibodies are commercially available and can be used for western blot analysis to detect exosome-associated proteins.
Or check for the other markers to confirm.
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