Mounipriya Tamma

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Questions related from Mounipriya Tamma

Does the higher quantity protein effects the smaller quantity protein during western blot antibody probing?

Dear all, I am working on identification of exosome related protein markers through western blot antibody probing. In my samples, there is an impurity seen at 66KDa which is obtained during the...

20 June 2023 9,325 2 View

What will be the problem during albumin purification in Tangential Flow Filtration in exosome samples?

Dear all, I am working with exosome purification using Tangential Flow Filtration and concentrating the samples using 750KDa Hollow fibers modules. I don't want albumin to be present in my final...

01 June 2023 664 0 View

A clear visible band of the protein has disappeared on the PVDF blot after specific Antibody probing. Are there any specific reasons?

I am working on exosome characterization by identifying the CD markers through Western blotting. Recently in one of the experiment I have come across a strange problem in the transferred PVDF...

01 May 2023 5,640 4 View