Hi there,
I am working on plant miRNAs using Next-Generation-Sequencing data. I have found in literature that some studies indicate a high correlation between expression of mature miRNA and its precursor, while others suggest a weak correlation.
I am wondering that, in a given sample (or transcriptome) collected from a given time, is the expression level of a given mature miRNA always positively correlated with that of its pri/pre miRNA?
I would be really appreciated if anyone who are working on this could advise me.
Hi,
It is just like the relation between mRNA and protein levels; they correlate roughly in general, but some post-transcriptional activities can make the difference between them.
- Hello Voang, you picked up right point. i am also working on tobacco micro RNA , In my personal experience, if this the cause ( abundance of a mature miRNA always positively correlate with that of its pri/pre miRNA) then you would always get same expression of pri/pre miRNA in northern blot while your looking the status of mature micro RNA but this is not the case. Hope this observation might help you.
Hi,
It is just like the relation between mRNA and protein levels; they correlate roughly in general, but some post-transcriptional activities can make the difference between them.
When northern blotting I have seen some miRNAs that have higher expression of the precursors than the mature miRNA and visa versa.
So I think it varies between miRNAs, it may also suggest that not all precursors continue to become a mature miRNA.
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