Hi Iván,

we have used PAXgene. They work fine. Only three comments:

1. you should spin down the sample only once. Otherwise you will lose your sample.

2. you con not do any cell separation after PAXgene

3. the whole blood amount you can use per PAXgene tube is pretty low (2ml). On case of RNA analysis make sure that this will end in a sufficient quantety of RNA.

Good luck

Michael

Thank You so much Jan-Michael for Your answer,

1. In which step I should spin the sample down only once?

2. If I just want to extract total RNA from the whole blood the fact that I can not do any cell separation after PAXgene should not be a problem in my case, should be?

3. Based on your own experience, which is the yield or RNA quantity/conc, for every 2 ml of whole blood?

Thank You again for Your time,

Ivan

Hi Ivan

It works quite well, but you have to use the PAXgene Blood RNA kit for the extraction (a "little" expensive) and not your own protocol. You should get  3µg of total RNA for 2mL.

Best wishes

Isabelle

Hi Isabelle,

Thanks for your reply. Do you know, or someone Knows, whether it's possible to collect the whole blood with these special columns and proceed to the RNA extraction and purification afterwards? I mean after the transport from the hospital to the lab which takes about 2 hours? I would still have intact RNA after that time? Does the transport needs any special conditions?   The whole blood has to be extracted directly from the patient in a way that is directly collected into the special collection tubes with the RNA special stabilization buffer? Or it can be transferred from a previous standard blood collection tube to the special one?

Thank You so much again for Your time and help,

Best wishes,

Ivan

Which Reverse Transcription Kit Do You use? Anybody use Omniscript or the QuantiNova from QIAGEN? Which one would be better for this purpose?