Iván Domènech Mercadé

7 Questions 5 Answers 0 Followers

Questions related from Iván Domènech Mercadé

Problems with BL21(DE3) pLysE bacteria transformation?

I am trying to transform the BL21(DE3) pLysE Chemically Competent Cells with my bacterial plasmid showed bellow which is Ampicilin resistent and expressing my genes of...

04 June 2018 1,670 7 View

Anyone has the faintest idea of why could be due to this strange and funny band shape on my agarose gel?

I performed a reverse PCR from a cDNA plasmid template in order to skip one exon of my gene of interest. Product size seems the correct one (5.7 Kb). But I don't know the reason why the bands on...

19 October 2017 5,738 10 View

Does anyone have experience splitting COS-1 Cells in 150 mm (10 cm) dish?

I would like to know which volumes You use to split a 100 % confluent 150 mm COS-1 cells dish in order to obtain an 80% confluent 150 mm dish 24 h after splting. Which volumes of tripsine do You...

20 July 2017 9,696 3 View

I'd like to unravel the Exonization or alternative splicing mechanism that cause or regulates differential isoform transcription in the same gene.

Isoform 1 of my gene of interest encondes a 323aa protein while isoform 2 differs in the 5' UTR, lacks multiple 3' coding exons, and includes a different terminal exon compared to isoform 1 (16 AA...

15 May 2017 140 2 View

In order to asses LOH, which is the maximum accepted distance between the microsatellite marker used and the putative LOH locus? Is 200 KB too far?

I manually sequenced and covered a wide genomic region for one specific patient and all it's SNPs within this region are homozygous. While in the other hand all the other samples have...

06 April 2016 4,669 1 View

What power conditions (volts or mA) should I use in Western blotting for transferring a 15.6 kDa protein onto a 0.45 micron PVDF membrane?

The thing is that I am transfecting both protein isoforms separately in 10 cm dishes with COS-1 cells and FuGENE® HD Transfection Reagent. I am transfecting 3 µg of the each vector containing each...

21 March 2016 5,460 11 View

There is any way of calculating the probability of having LOH? e.g.Taking into account the number of homozygous SNP's within the genomic size covered?

I manually sequenced and covered a wide genomic region for one specific patient and all it's SNPs within this region are homozygous. While in the other hand all the other samples have...

24 February 2016 4,264 4 View