01 June 2018 1 2K Report

I need to transfect the hIPSC with GFP. However, I am having a hard time to find a protocol of lentiviral transduction.

More Cristiano Pedrozo Vieira's questions See All
I have a problem with ATCC media does anyone use these product?

Hi to all!! I have prepared a media with these ATCC product: RPMI-1640 Medium 30-2001 L-Glutamine Solution, 200 mM 30-2214 Fetal Bovine Serum (FBS) 30-2021 Unfortunately I have aliquoted the...

05 December 2023 2,421 0 View

Does anyone know how to solve the VINA problem found in YASARA?

I recently started working with the YASARA software and I'm doing molecular docking. However, even using a small protein, I get an error that says VINA was unable to run successfully. In the user...

09 October 2023 2,679 8 View

I'm looking for articles with faunal list at the time of H. neanderthalensis, in specific sites. Any help?

I need to discover the actors of ecosystem where Neanderthal lived, in order to better understand his role in that ecosystem. Datation near -40ka are preferred. I can use reviews that list the...

08 May 2023 4,300 3 View

What can trigger precipitation over oceanic regions?

Precipitation over continents can be triaged by orography, dirunal heating, mesoscale convergence and etc... On the other hand, those kinds of heterogeneities are mostly absent over the ocean....

08 January 2022 5,221 3 View

Inter-trial coherence (ITC) of EEG data in EEGLAB?

How to compute/plot inter-trial coherence in EEGLAB for all participants (e.g. averaged) data pooled? Is there any tutorial available? Advices are also welcome, currently I'm able to do this only...

05 November 2021 4,353 5 View

Sensibility of GC-HRMS for analysis of dioxins and furans is changing with sample injection. Any idea about what could be possibly happening?

I am currently working with analysis of dioxins, furans and PCBs using a GC-HRMS from Thermo (DFS) and I'm having problems with the detection of some dioxins and furans. What happens is it: I...

06 June 2021 8,021 7 View

Concentration of ions of MM-PBSA binding energy calculation?

Hi, I ran a DM simulation of a system with enough ionic concentration to neutralize the system (5 Na added to 1 structural NaCl). Now I want to do an MM-PBSA analysis between protein and ligand...

12 April 2021 6,408 2 View

Is there a way to cancel/delete a bankit submission?

Hi, I've submitted some sequences with incorrect metadata. Already sent an email to NCBI. However, it would be way faster if I could just delete the records and resubmit them. Thank you very...

09 December 2020 7,896 1 View

Best algorithm to tracking soccer ball?

I'm looking for an algorithm to help automatically track soccer ball in a controlled field experiment (cameras positioned near to the ball) and output its time-series 2-dimensional coordinates....

22 November 2020 8,533 5 View

Can I fix the citation of me as main author in my recent article on Google scholar ?

Something wrong with my status authorship on my recent article when your Team has linked on Google scholar

22 September 2020 1,663 4 View

Similar questions and discussions
Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close