Can I fix the citation of me as main author in my recent article on Google scholar ?

Something wrong with my status authorship on my recent article when your Team has linked on Google scholar

22 September 2020 1,526 4 View

Nanoparticles and microfluidics setup ?

Hi to all! I'm approaching to the microfluidics system for nanoparticles production and I have some question about the basic setup I need to buy. Until now I've used liposomes and polymeric...

03 September 2020 3,221 4 View

Which statistical test when comparing 2 cell groups over time?

I need to compare the transport of a compound across 2 different cell lines (non-transfected vs. transfected) over time. My goal is to compare the mean transport between Cell 1 and Cell 2 at time...

08 July 2020 2,410 5 View

How to create a Lab in RG?

24 June 2020 561 11 View

What are the convergences and differences between critical and creative thinking?

There are different definitions and references for these two types of thinking. However, the research that has been carried out and that is being disseminated common points and, in many cases,...

29 January 2020 2,342 8 View

How can I do thin-films of DNA by spin coating?

I have salmon testis DNA in which I will disperse R6B dye molecule for fluorescence. My question is wherter I can dissolve the DNA in water or ehtanol and spin coat on silver sulbstrate.

30 January 2019 7,437 2 View

Does anyone know where I can access a copy of the JPED Japanese pharmaceutical excipients in english. I am looking for the most recent version?

Does anyone know where I can access a copy of the JPED Japanese pharmaceutical excipients in english. I am looking for the most recent version?

21 January 2019 5,008 1 View

How do you understand the relationship between problem solving and the development of higher order thinking skills (HOTS) in mathematics teaching?

I am looking for convergences between working with problem solving and the development of HOTS.

28 November 2018 5,549 3 View

Can someone share a DNA extraction protocol for ants (Hymenoptera: Formicidae) that yields DNA that can be used for Illumina sequencing?

I need to extract DNA from ants for posterior Illumina sequencing, but I do not know which extraction protocol would be fit for the task. After reading Gadau (2009), I'm considering the use...

26 April 2017 5,430 2 View

Does anyone know any stain kit for labeling the nucleus in an immunocytochemical analysis ?

Does anyone know any stain kit for labeling the nucleus in an immunocytochemical analysis? Something like NucRed Live 647, but close to 594nm peak excitation. No UV please, we do not have UV in...

06 February 2017 7,164 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View