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I need to transfect the hIPSC with GFP. However, I am having a hard time to find a protocol of lentiviral transduction.
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Which concentration of Matrigel you use for iPSC culture? I was using 500 ul in 15ml and then, I have added 2 ml in 6 cm dish. However, my colonies did not attach. Now, I am using 1.5ml of...
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