19 October 2022 1 4K Report

Does anyone know how to use the Sholl Analysis tool by Image J (Fiji) to calculate the neurite lenght?

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How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Problem Justifcation of below research topic?

Analyzing the effectiveness of apply the Extended Producer Responsibility (EPR) concept for reducing pre-consumer apparel waste within a Star Fashion Pvt Ltd apparel company.

30 June 2024 3,764 0 View

Anyone familiar with the Bruker in-vivo F-PRO Photodocumentator?

Hello! I'm a PhD student and we need urgent technical help for the "Multimodal Pro Light Source 400W" equipment. We are having image acquisition problems in our laboratory. When we activate...

16 May 2024 9,545 0 View

Critically discuss complexity theory and the notion of resilience as they relate to public policy?

Explain the concept of complexity theory Discuss the link between resilience and complexity theory Explain how these relate to public policy illustrate your discussion with appropriate example

05 April 2024 8,886 1 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What evaluate to choose the best docking program for my protein and ligands?

I performed virtual screening of some molecules in Auto Dock Vina and GOLD and obtained different results for the best ligand. I'm not sure if I did something wrong, or the docking program is not...

07 March 2024 3,680 3 View

How to separate a misture of coordenation coumpounds that are water-soluble?

I did a synthesis of a carbonyl ruthenium complex coordinated with the 1H-imidazole-1-ylacetic acid, but I'm having problems in the purification process. All the mixture is only slightly soluble...

03 March 2024 2,041 3 View

Continuing PCR with contaminated reagents?

I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...

21 February 2024 4,799 0 View

Can the same primers be used for different cell lines from the same species?

If primers amplify a specific region of DNA, and the same DNA is present throughout the body, can a single primer be used across different cell lines and different organs from the same species?

23 January 2024 6,465 2 View

Similar questions and discussions
Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

How can I better use imageJ to get good result on calculating surface area of a film accurately?

I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...

23 July 2024 4,461 1 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

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