Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Why was sonication unsuccessful for 1 of 6 samples?

I have sonicated six HeLa-cell samples in RIPA lysis buffer (prior inc. on ice, 15 min). For one of these samples, the sonication was unsuccessful. My question is what factors should be taken into...

17 December 2020 8,531 2 View

Maskning for polysterene substrate?

We want to deposite a fine pattern ( 100-300 nm thick and 10 microns wide pattern) of Pt as temperature sensors on a polysterene substrate with a glass temperature of 95 degrees C. How would...

12 November 2020 9,761 3 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

Barriers and Factors with wound are discharge planning, how to narrow down this topic?

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down. Any ideas on wounds that need more...

29 May 2020 8,870 1 View

Why are some plasmids (such as pKD46) temperature sensitive? What happens at higher temperatures to cause plasmid loss?

I am trying to figure out how temperature sensitive plasmids are made/isolated and what the biochemical/cellular mechanism is that causes them to be lost/lose function at higher temperatures.

20 March 2020 6,815 3 View

In 6-line Ferrihydrite synthesis is dialysis necessary or can the salt be removed with washing?

I don't have dialysis tubing and am wondering if washing is sufficient.

09 March 2020 5,484 3 View

What would be the best method to attach a radio transmitter to a grey-headed robin?

I am planning to use radio telemetry in a study of grey-headed robins (Heteromyias cinereifrons). These birds weigh around 35 g and I have sourced glue-on transmitters weighing 0.9 g (

09 December 2019 3,623 3 View

Where can I find the KIDCOPE sacles for both children and adolescence, along with scoring guidance?

The KIDCOPE scales are widely used to measuring coping in children and adolescents. There is a lot of literature using them, or assessing them, but nowhere can I find the actual scales and...

03 December 2019 4,451 2 View

How do I make a map in QGIS of a area to get GPS coordinate when no signal is available?

Th question above explains most of what I am looking for. I have been given a great opportunity for a field project but I am only just now learning of QGIS and I have a bit of a time constraint....

08 October 2019 2,389 5 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View