Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Why was sonication unsuccessful for 1 of 6 samples?

I have sonicated six HeLa-cell samples in RIPA lysis buffer (prior inc. on ice, 15 min). For one of these samples, the sonication was unsuccessful. My question is what factors should be taken into...

17 December 2020 8,531 2 View

Maskning for polysterene substrate?

We want to deposite a fine pattern ( 100-300 nm thick and 10 microns wide pattern) of Pt as temperature sensors on a polysterene substrate with a glass temperature of 95 degrees C. How would...

12 November 2020 9,761 3 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

Barriers and Factors with wound are discharge planning, how to narrow down this topic?

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down. Any ideas on wounds that need more...

29 May 2020 8,870 1 View

Why are some plasmids (such as pKD46) temperature sensitive? What happens at higher temperatures to cause plasmid loss?

I am trying to figure out how temperature sensitive plasmids are made/isolated and what the biochemical/cellular mechanism is that causes them to be lost/lose function at higher temperatures.

20 March 2020 6,815 3 View

In 6-line Ferrihydrite synthesis is dialysis necessary or can the salt be removed with washing?

I don't have dialysis tubing and am wondering if washing is sufficient.

09 March 2020 5,484 3 View

What would be the best method to attach a radio transmitter to a grey-headed robin?

I am planning to use radio telemetry in a study of grey-headed robins (Heteromyias cinereifrons). These birds weigh around 35 g and I have sourced glue-on transmitters weighing 0.9 g (

09 December 2019 3,623 3 View

Where can I find the KIDCOPE sacles for both children and adolescence, along with scoring guidance?

The KIDCOPE scales are widely used to measuring coping in children and adolescents. There is a lot of literature using them, or assessing them, but nowhere can I find the actual scales and...

03 December 2019 4,451 2 View

How do I make a map in QGIS of a area to get GPS coordinate when no signal is available?

Th question above explains most of what I am looking for. I have been given a great opportunity for a field project but I am only just now learning of QGIS and I have a bit of a time constraint....

08 October 2019 2,389 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View