Does semen preservation in liquid nitrogen without cryoprotectant affect it's quality? how can i prepare a cryoprotectant?

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Georgi Lazarov

I'm sorry, but this is a matter far beyond my expertise

Humberto O Cisale

Definitely, not using a diluent to preserve sperm morphology and functionality will affect its conservation possibilities. In this sense, a number of cryoprotective media and cryopreservation methods have been developed, that have given different results in the preservation of sperm from various species. The variety is such, and the sperm behavior of each species is so different, that it is impossible to design a combination cryoprotectant diluent - cryopreservation process unique for all species. Therefore, first of all, to help you, we must know what species you are referring to, and accordingly send you to the appropriate bibliography for the case, which, in general, is extensive for slaughter animals.

Hope it helps.