Does S. aureus virulence decrease after incomplete freeze/thaw cycles?

26 January 2018 1 3K Report

Has anyone had anecdotal or experimental experience of S. aureus decreasing in mouse virulence after using the same glycerol stock for streaking out plates for inoculum growth?

Sourav Maiti

In some instances I had noted this. However I have not investigated further.

Badges
Science topic

More Ida Lister's questions See All

How to avoid gelation/slime formation in muscle homogenate?

I am isolating mitochondria from rat quadriceps, using the following procedure: First I cut ~200 mg quadricep placed in 1 mL of ice-cold isolation buffer (Sucrose 100 mM, KCl 100 mM, Tris–HCl...

21 March 2024 2,132 0 View

Could you please remove the full version of our last article?

Could you please remove the full version of our last article with co-author Szilvia Vanessza Nador: Opportunities for the hotel industry in the United States to recover from COVID -19: a...

12 March 2023 1,201 4 View

Antisera for AGRP- and POMC-neurons with success on fresh-frozen non fixed tissue?

I look for advise and suggestions of antisera for AGRP- and POMC-neurons in mouse with success on fresh-frozen non fixed tissue? Postfixation of tissue sections can be included in the protocol

24 October 2022 1,606 1 View

How can I use vacuum desiccator as an evacuation-replacement system for bacteria growth?

I would like to growth anaerobic bacteria, but do not have anaerobic chamber. How Can I use vacuum desiccator as an evacuation-replacement system?

07 August 2022 9,014 2 View

Mice don’t recover after CLP?

i tried performing a CLP procedure to induce sepsis in mice and i followed all the instructions in advertised protocols and videos. after performing the procedure, even a successfull and clean...

04 December 2021 441 5 View

Brief Job Stress Questionnaire?

Does anybody have the items of "Brief Job Stress Questionnaire"? Many thanks,

24 October 2021 8,372 6 View

How to analyze parallel multiple mediation with Smartpls?

I want to analyze 1IV, 1DV and 4 mediator. I struggle to find references about it. Thank you so much, Ida

16 September 2021 1,529 3 View

Any study on role of leisure in the life of caregivers of autism spectrum disorder?

As leisure plays major role in psychological health of caregivers i would like to know if any study done to understand the need of leisure in their life.

13 August 2021 6,339 4 View

When checking on distribution of residuals among multiple groups of a variable, what is the cut-off to use nonparametric test? Is there a citation?

In the past, I used a 20% cut-off as a rule-of-thumb when making decision about use or not to use parametric procedure on a numerical response variables. For example, there would be a 3-factorial...

27 June 2021 9,639 0 View

How is it possible to create a new department in an institution?

I would like to update to an official department, called ICT Research Centre (I'm the head of this deprartment), but I can't update it. How is it possible? My institute is Károli Gáspár...

24 March 2021 4,517 6 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Suggestions for storage of CD4+ naive splenocytes for future use?

Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...

02 July 2024 4,887 0 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View