Is there any books or journal that specifically discussed TPR in detail?

I am designing a metal oxide catalyst and among many characterization of this sample, TPR emerges quite significantly. The brief explanation in journal is limited to the sample that is...

21 January 2021 6,346 1 View

In a Contingent Valuation (CV) study, does asking questions before or after the DBDC CV - section matter?

I'm working on an agricultural technology project now. We used a double-bounded dichotomous choice contingent valuation approach to assess consumer's willingness-to-pay (WTP). The study had four...

15 October 2020 5,495 3 View

How to calculate contribution of each emission sector to atmospheric concentration of green house gasses?

If we measure atmospheric greenhouse gases for instance CH4 (methane) using CRDS instrument, how we can measure contribution emission CH4 from vehicle, rice paddy, wet land, farm etc, and how...

24 February 2020 3,535 3 View

Has the Avant-Garde become intitutionalized? How can students learn to adopt an attitude?

Is the art world guided by "an invisible hand"- or has it become "economics of regulation"? The problem in art was the turn around the sixties:- controversial art became investment -that makes the...

10 July 2017 9,881 1 View

Could NS-3 be used to simulate Analog Network Coding (ANC) or Physical-Layer Network Coding (PNC) in mesh wireless networks?

I am in a project to simulate how Analog Network Coding(ANC) or PNC works in Mesh Wireless Networks. Is it possible to simulate ANC or PNC in NS-3? I know there is KODO library for network coding...

20 February 2017 6,135 3 View

Is there any specific volume percentage (w/v) to buffer for lysing the bacterial pellet ?

Recently, i'm doing a sonication to bacterial pellet using PBS 10x buffer. I'm being confused in adding the buffer to the pellet for sonication. I'm setting the amplitude for 20-30%. Is there any...

27 October 2016 8,877 2 View

Why using the whole-cell biosensor gives a fluctuative signal when detecting AHL ?

I've been working with E.coli JM109 pSB401 for detecting the C8-HSL from Burkholderia cenocepacia species. The AHL was extracted using Acidified Ethyl-acetate and then suspended into Methanol...

27 October 2016 6,163 1 View

Does 0,1 M PBS buffer could be used for cell lysis through sonication method ?

Recently, I'm trying to lyse the bacterial pellet with sonication to get the protein. Before running the sonication, the pellet was re-suspended into 0,1M PBS buffer. After running the sonication,...

11 October 2016 9,852 6 View

I'm running MS-HRM PCR for Nasopharyngeal Cancer marker, I have an issue with melting curve of control standart ?

I'm using 100% methylation & 0% methylation plasmid for standart control. Then, we mix them to get 75%, 50%, 25% & 10% standart. Then, we convert with BIsulfite modification and run in...

06 October 2016 522 1 View

Dear, why in HPLC method with detector UV-Vis not appear peak in chromatogram in wavelength maximum?

In my research, to check purity of my synthesized compound I use HPLC method with detector UV-Vis. So, first I did spectroscopy UV my compound and appear two peaks maximum (237 & 367 nm) ....

15 May 2016 6,989 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View