Willy Kurniawan

5 Questions 2 Answers 0 Followers

Questions related from Willy Kurniawan

Why is the sludge of waste water so viscous eventough the MLSS value is still low?

I have an project for treat the waste from tea factory, mainly the process is biological treatment which used aeration & MBR (membrane bio-reactor). The MLSS is still around 5.000 mg/L but the...

13 October 2017 5,765 3 View

Does quorum quenching treatment trigger/cause the resistance from bacteria ? ?

I've been working with quorum quenching molecule, such as the acylase & lactonase enzyme for degrading the AHL molecule from B.cenocepacia species. Is it true that this quorum quenching...

06 January 2017 3,850 5 View

Why using the whole-cell biosensor gives a fluctuative signal when detecting AHL ?

I've been working with E.coli JM109 pSB401 for detecting the C8-HSL from Burkholderia cenocepacia species. The AHL was extracted using Acidified Ethyl-acetate and then suspended into Methanol...

28 October 2016 6,238 1 View

Is there any specific volume percentage (w/v) to buffer for lysing the bacterial pellet ?

Recently, i'm doing a sonication to bacterial pellet using PBS 10x buffer. I'm being confused in adding the buffer to the pellet for sonication. I'm setting the amplitude for 20-30%. Is there any...

28 October 2016 9,000 2 View

Does 0,1 M PBS buffer could be used for cell lysis through sonication method ?

Recently, I'm trying to lyse the bacterial pellet with sonication to get the protein. Before running the sonication, the pellet was re-suspended into 0,1M PBS buffer. After running the sonication,...

12 October 2016 9,943 6 View