When apoptosis is initiated, procaspases is converted to the caspase. So does the procaspases is available from before or it is transcribed after apoptosis is initiated?
I read an interesting article from Christensen (2007): A Monte Carlo approach to unidimensionality testing in polytomous rasch models. But do you have some further thoughts or maybe other...
08 August 2024 4,245 0 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,500 3 View
The adsorbate is Arsenic and the adsorbent is a soil
18 July 2024 332 8 View
I am using a polygon mirror scanner that have 6 facets and the dc brushless motor speed was given 10,000 RPM. Now I want to know how to calculate the polygon speed if I change the power supply...
11 July 2024 5,942 1 View
I'm attempting to simulate a crossed dipole antenna. For excitation, I've placed a lumped port along each dipole. However, the resulting radiation pattern is similar to that of a single dipole....
08 July 2024 1,876 3 View
Does anyone know if there is a lab scale encapsulation machine available for purchase?
05 July 2024 3,888 1 View
I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...
01 July 2024 7,104 1 View
I am doing md simulation for protein aggregation. For doing cluster analysis I was trying with gmx clustsize. Now the plot that I am getting, can not find a proper explanation. With -hc flag the...
24 June 2024 5,246 0 View
if yes, is the publication free of charge
06 June 2024 4,270 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...
13 July 2024 356 3 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
08 July 2024 5,482 2 View
Greetings mathematicians and physicists, As we know, the idea of assuming a higher space dimension than the natural 3 dimension is effective in solving many physical and mathematical...
29 June 2024 9,220 6 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
I am using SU-8 2025 to generate a high aspect ratio microchannel. I made two microchannels with the properties of: 1. width = 10 um. height = 25 um. SU - 8 2025. 2. width = 15 um. height = 37.5...
27 May 2024 6,862 2 View