Hi everyone
I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF.
Question is whether transcardial perfusion artefactually stretch the rodent heart? Because I observed thinner (?stretched) chambers when compared to hearts of unperfused mice.
The transcardial perfusion was done without perfusion machine by inserting needle/syringe into left ventricle and snipping the right aorta. 10mL of heparin/PBS was injected followed by 10mL of 4% PFA.
I guess it depends on the pressure and time used to perfuse. What did you perfuse it with? Fixative? Or, buffer?
Daniel Carneiro Moreira Hi Daniel! Thanks for the reply.
The transcardial perfusion was done manually by inserting 22G needle attached to a 10ml syringe into left ventricle and snipping the right aorta.
As such the perfusion pressure and time was not taken into consideration. It usually take me about 5 min to inject 10mL of fluid.
10mL of Heparin/PBS was first injected followed by 10mL of 4% PFA.