6 Questions 10 Answers 0 Followers
Questions related from Malcolm Lim
Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...
29 October 2020 8,246 2 View
Hello I am looking for a software which allows me to annotate tumour regions, count the number of foci (spots) and nuclei. These are breast carcinoma TMA slides stained immunofluroscently using...
30 November 2016 2,471 4 View
I use the pressure cooker for antigen retrieval. Citrate buffer:125 deg for 5m then 90deg for 10m, EDTA buffer: 105 deg for 15m then 90deg for 10m. Just wonder if the heating profile can be same...
27 October 2016 2,151 2 View
Any one has tips on frozen sectioning fatty tissues e.g. Margins, lymph nodes? The high fat content made it very very challenging to get a good section. secondly, If you have tips on cutting Fatty...
02 March 2016 7,273 3 View
Gene discovery usually leads to knock down studies etc and one will usually end up requiring to test and optimise the antibody for the protein in question. Currently, I have western blots to test...
21 December 2015 620 3 View
First attempt on IF, I did HER2 on breast ca cells. Cytoplasmic stain (green) observed instead of membraneous as per literature. Im not sure if this cytoplasmic staining is real or am I not using...
09 December 2015 6,083 9 View