Malcolm Lim

6 Questions 13 Answers 0 Followers

Questions related from Malcolm Lim

Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

29 October 2020 8,358 2 View

Software suggestions for counting foci on IF slide?

Hello I am looking for a software which allows me to annotate tumour regions, count the number of foci (spots) and nuclei. These are breast carcinoma TMA slides stained immunofluroscently using...

30 November 2016 2,517 4 View

Pressure cooker Antigen retrieval, Same Heating profiles for ALL buffers?

I use the pressure cooker for antigen retrieval. Citrate buffer:125 deg for 5m then 90deg for 10m, EDTA buffer: 105 deg for 15m then 90deg for 10m. Just wonder if the heating profile can be same...

27 October 2016 2,202 2 View

Any tips on frozen section of fatty adipose tissues?

Any one has tips on frozen sectioning fatty tissues e.g. Margins, lymph nodes? The high fat content made it very very challenging to get a good section. secondly, If you have tips on cutting Fatty...

02 March 2016 7,315 3 View

How do I optimize IHC antibody?

Gene discovery usually leads to knock down studies etc and one will usually end up requiring to test and optimise the antibody for the protein in question. Currently, I have western blots to test...

21 December 2015 694 3 View

Is my cytoplasmic signal real with my Confocal Microscopy?

First attempt on IF, I did HER2 on breast ca cells. Cytoplasmic stain (green) observed instead of membraneous as per literature. Im not sure if this cytoplasmic staining is real or am I not using...

09 December 2015 6,133 9 View