Does one 12well transwell insert provide enough protein cell lysate for WB?

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Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

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I'm just learning the ePrime 3.0 software and have created a test. How can I put this test on a tablet for subjects to use for data collection?

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Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

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Inconsistent protein expression for different maxipreps?

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Who is investigating artificial intelligence / machine learning for leadership augmentation and resource distribution?

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Could you recommend a scale that measures group intelligence or the predisposition to work in a group?

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Could you kindly assist with the journals that are related to my title below?

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A.B Bayazid

Dear Rebecca Miller

12-well plate is enough for WB analysis. As you know the protein conc. will very on cells type.

Briefly, After 1) Fixing by ice-cold PBS, 2) a. add 20-50uL of lysis buffer and run it thoroughly, 3) keep it dark at 4C over an hour then 4) centrifuge and collect the supernatant.

2) b. or you can collect cells into e-tube then add lysis buffer (ProPrep/RIPA)