Does one 12well transwell insert provide enough protein cell lysate for WB?

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A.B Bayazid

Dear Rebecca Miller

12-well plate is enough for WB analysis. As you know the protein conc. will very on cells type.

Briefly, After 1) Fixing by ice-cold PBS, 2) a. add 20-50uL of lysis buffer and run it thoroughly, 3) keep it dark at 4C over an hour then 4) centrifuge and collect the supernatant.

2) b. or you can collect cells into e-tube then add lysis buffer (ProPrep/RIPA)

Rebecca Miller

Thank you!