Benefits of accommodation establishments
I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).
09 February 2021 314 7 View
Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...
07 February 2021 3,643 4 View
I am trying to do a thermal shift assay for a recombinantly-produced membrane protein, by western blot band quantification. I use Bio-rad 10X Tris/glycine SDS running buffer for my SDS-PAGE and...
11 January 2021 5,302 2 View
I am genuinely intrigued by the few papers and articles that I have read so far regarding Disaster Management Framework. I have yet referred to any specific researchers and have yet managed to...
07 December 2020 1,325 8 View
I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...
05 December 2020 9,014 3 View
I have a flag-tagged protein and want to be sure it is properly expressing in my cells. When I assess by IF, I see clear nuclear enrichment (expected). But when I do a western, there is only a...
27 November 2020 2,350 3 View
Effects of covid-19 pandemic on micro companies within the event sector in Western Cape.
08 November 2020 2,138 2 View
Corona significantly changes human life. I would like to discuss how this change will influence ethical tourism considering consequences of actions as a tourist on the environment, local people...
20 October 2020 4,198 9 View
Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...
06 October 2020 1,107 5 View
Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...
19 September 2020 538 7 View
