Does N9 microglia cell line have serotonin/norepinephrine transporters?
Hi Everyone, I have tried to run pure antibody (55kD) in 10% SDS/PAGE. I have seen three bands at 250 kD, 75 kD and 50 kD respectively. There is no reducing agent added in antibody. I'm wondering...
08 September 2017 7,950 11 View
How can I measure NLRP3 inflammasoem activation? In most of the papers, I found protein and mRNA expression levels of NLRP3 and caspase-1. Since NLRP3, Asc and procaspase-1 make an assembly and it...
02 March 2017 2,578 5 View
Can anyone suggest to me where i can buy N9 microglia cell line?
10 November 2016 7,895 2 View
Maximum concentration of Tween-20 in TBS-T in immunoblot technique??
08 September 2016 6,403 3 View
I'm looking for 12 kD protein expression by SDS/PAGE. The bands I was observing earlier were single crisp bands. Few days back I have started seeing fuzzy bands. I didn't make any changes in...
04 May 2016 4,890 9 View
I'm trying to detect total S-nitrosylation in SH-SY5Y cell culture using Sodium Nitroprusside (200, 400uM). I've treated cells with SNP for 15 min and isolated proteins using lysis buffer...
31 December 2015 10,114 0 View
Please correct me if you have tried any other method or reagents. 1) 12% separating gel (5ml): 30% acrylamide: 4ml, 0.375 M Tris-HCl (pH=8.8): 5.89 ml, 10% APS = 100 ul, TEMED= 10ul 2) Stacking...
08 September 2015 867 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
