Pure antibody in western blot is showing three bands. What does these bands represent?

Hi Everyone, I have tried to run pure antibody (55kD) in 10% SDS/PAGE. I have seen three bands at 250 kD, 75 kD and 50 kD respectively. There is no reducing agent added in antibody. I'm wondering...

08 September 2017 7,860 11 View

Does N9 microglia cell line have serotonin/norepinephrine transporters?

Does N9 microglia cell line have serotonin/norepinephrine transporters?

04 May 2017 8,110 0 View

How to measure NLRP3 inflammasome activation?

How can I measure NLRP3 inflammasoem activation? In most of the papers, I found protein and mRNA expression levels of NLRP3 and caspase-1. Since NLRP3, Asc and procaspase-1 make an assembly and it...

02 March 2017 2,472 5 View

Maximum concentration of Tween-20 in TBS-T in immunoblot??

Maximum concentration of Tween-20 in TBS-T in immunoblot technique??

08 September 2016 6,281 3 View

Fuzzy and two bands instead of single crisp band in SDS/PAGE?

I'm looking for 12 kD protein expression by SDS/PAGE. The bands I was observing earlier were single crisp bands. Few days back I have started seeing fuzzy bands. I didn't make any changes in...

04 May 2016 4,729 9 View

How can I detect total s-nitrosylation in cell culture?

I'm trying to detect total S-nitrosylation in SH-SY5Y cell culture using Sodium Nitroprusside (200, 400uM). I've treated cells with SNP for 15 min and isolated proteins using lysis buffer...

31 December 2015 9,977 0 View

Can anyone suggest NAtive PAGE gel conditions?

Please correct me if you have tried any other method or reagents. 1) 12% separating gel (5ml): 30% acrylamide: 4ml, 0.375 M Tris-HCl (pH=8.8): 5.89 ml, 10% APS = 100 ul, TEMED= 10ul 2) Stacking...

08 September 2015 751 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View