Veni Bharti

8 Questions 6 Answers 0 Followers

Questions related from Veni Bharti

Pure antibody in western blot is showing three bands. What does these bands represent?

Hi Everyone, I have tried to run pure antibody (55kD) in 10% SDS/PAGE. I have seen three bands at 250 kD, 75 kD and 50 kD respectively. There is no reducing agent added in antibody. I'm wondering...

09 September 2017 7,916 11 View

Does N9 microglia cell line have serotonin/norepinephrine transporters?

05 May 2017 8,176 0 View

How to measure NLRP3 inflammasome activation?

How can I measure NLRP3 inflammasoem activation? In most of the papers, I found protein and mRNA expression levels of NLRP3 and caspase-1. Since NLRP3, Asc and procaspase-1 make an assembly and it...

03 March 2017 2,538 5 View

Can anyone suggest to me where i can buy N9 microglia cell line?

11 November 2016 7,860 2 View

Maximum concentration of Tween-20 in TBS-T in immunoblot??

Maximum concentration of Tween-20 in TBS-T in immunoblot technique??

09 September 2016 6,365 3 View

Fuzzy and two bands instead of single crisp band in SDS/PAGE?

I'm looking for 12 kD protein expression by SDS/PAGE. The bands I was observing earlier were single crisp bands. Few days back I have started seeing fuzzy bands. I didn't make any changes in...

05 May 2016 4,849 9 View

How can I detect total s-nitrosylation in cell culture?

I'm trying to detect total S-nitrosylation in SH-SY5Y cell culture using Sodium Nitroprusside (200, 400uM). I've treated cells with SNP for 15 min and isolated proteins using lysis buffer...

01 January 2016 10,066 0 View

Can anyone suggest NAtive PAGE gel conditions?

Please correct me if you have tried any other method or reagents. 1) 12% separating gel (5ml): 30% acrylamide: 4ml, 0.375 M Tris-HCl (pH=8.8): 5.89 ml, 10% APS = 100 ul, TEMED= 10ul 2) Stacking...

09 September 2015 827 5 View