Maximum concentration of Tween-20 in TBS-T in immunoblot??

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Bhupesh Singla

We normally use 0.1%.

Jekaterina Muhhina

In general Tween concentration is 0.05%-0.2%

Veni Bharti

We use 0.5% concentration of Tween-20 in our lab. But we have to use one of the primary antibody in higher concentration in order to get clear bands but that gives a high background. I'm not sure if increasing Tween-20 conc. will help to reduce the background?