Does lambda red recombination technique work for organisms other than E.coli as well?

Alazhar Colombowala @Alazhar-Colombowala

04 July 2022 1 4K Report

I am trying to knock out a gene of interest and trying to understand different methods for it ... I am new to knockouts and therefore if anyone has any protocols or methods that I can follow would be of great help

Thank you.

Hanna Alalam

lamda red has been tested for other kind of organisms, mostly other gram negative bacteria. However, there are constrains on each tested species (E.X different length of homologies etc..). In other cases it does not work at all and what is used instead are RED homologs from species specific phages. You need to specify what you are working with so that it could be pointed out whether lamda red has been optimized for your species or not.

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