Alazhar Colombowala

5 Questions 3 Answers 0 Followers

Questions related from Alazhar Colombowala

Does lambda red recombination technique work for organisms other than E.coli as well?

I am trying to knock out a gene of interest and trying to understand different methods for it ... I am new to knockouts and therefore if anyone has any protocols or methods that I can follow would...

04 July 2022 3,851 1 View

Can anyone suggest detailed protocol for chemical estimation of siderophore nature ?

i am trying to find the nature of siderophore produced by the bacterial isolates am working with looking for an assay to detect its chemical nature however I have been trying arnows, cazky, fecl3...

21 January 2022 7,012 0 View

Can anyone tell me about the arnow's test ?

I am using 1ml of 0.5N HCL+1ml of nitrate molybdate reagent and getting yellow color. after I add 1ml culture supernatant the color still remains yellow, however when I add 1ml NaOH the color...

21 January 2022 3,750 0 View

Can siderophores be purified using amicron filters with a cut off of 10000MW?

i am trying to purify siderophores however looking for an alternative to column chromatography

19 January 2022 1,352 7 View

How to detect catecholate siderophore if i am not getting any color in arnow's test ?

I have tried several times to identify the siderophore produced by my organism however I am not able to do so. I have tried methods like arnow's test, fecl3 spectrophotometric detection test but I...

19 January 2022 7,471 2 View