Doing SVF from adipose tissue i'm using collagenase NB4 (serva). For inhibition collagenase i want use human ab serum (seracare). Does it work?
Hi everyone. I want to DNA isolate from transfected cell culture medium (transfected with lentivirus). I tried lots of kit but DNA cons and quality not very well and I didnt show at agarose gel.
19 March 2024 6,763 1 View
Hi everyone, I recently conducted a WST-1 assay on iPSC-derived cardiomyocytes and encountered unexpected results. I seeded cells into a 96-well plate at three different densities (Each sample...
12 August 2023 7,423 0 View
Hi everyone, I have a question regarding indirect FACS protocol. I'm using an unconjugated antibody which is biotin-tagged and PE-streptavidin as a secondary antibody. What should be their...
13 July 2023 4,603 3 View
I wanted to investigate the inflammatory response of the lipopolysaccharide from the bacterium Porphyromonas gingivalis (P.G.) on human gingival fibroblast cells. To try it out, I wanted to...
22 March 2022 3,123 1 View
I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...
12 October 2020 3,743 6 View
Hi everyone. In my experiment, I compared different passages (p2, p4 and p6) in one cell line in terms of the same genes and used GAPDH as a housekeeping gene. I have no control, I just compared,...
08 May 2020 5,482 0 View
we are planning to use Refrigerant (R-1234yf) gas and would like to have prediction to evaluation for measurements against explosion both explosive, flammable areas. So that flammability level is...
10 July 2019 9,101 2 View
Hi all, Like many of you, I was coating my Si wafer molds with a silanization agent (TFOCS) using vapor deposition technique to facilitate easy removal of PDMS. However, the last time, I...
02 May 2019 4,477 2 View
I have been designing a model of an already existing actuator which is run in FOC with an SVPWM inverter and cascade speed-current control. Losses like eddy current or hysteresis, or switching...
29 November 2018 2,323 4 View
Hello everyone, Like all of you know, endothelial cells align parallel to the direction of the flow as a response to flow shear stress. There are plenty of papers about this in the literature....
02 July 2018 6,042 17 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
what is the best research evidence for psychological interventions for Bipolar?
01 August 2024 6,023 2 View