Doing SVF from adipose tissue i'm using collagenase NB4 (serva). For inhibition collagenase i want use human ab serum (seracare). Does it work?
I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...
12 October 2020 3,583 6 View
we are planning to use Refrigerant (R-1234yf) gas and would like to have prediction to evaluation for measurements against explosion both explosive, flammable areas. So that flammability level is...
10 July 2019 8,943 2 View
Hi all, I was trying to model deflection in a rectangular cross-section beam fixed at both ends. There is a Pressure Load acting on the beam (uniformly distributed along the beam). In such...
25 November 2017 3,094 18 View
Hello everyone, I was trying to model concentration gradients in a flow-free diffusion chamber using COMSOL. I wanted see what would be the effect of gravity on the concentration gradients that I...
06 June 2017 2,759 3 View
I will do cell culture with specific patients PBMC. I isolated the PBMC and now will stimulate them with PHA as I want to see Th17 association with the disease. After stimulation I will take the...
26 December 2014 2,879 9 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
02 March 2021 7,082 3 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...
01 March 2021 6,200 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View