Hazal Sönmez

5 Questions 2 Answers 0 Followers

Questions related from Hazal Sönmez

Low DNA Yield and Poor A260/230 Ratio with QIAamp Fast DNA Stool Mini Kit – Any Suggestions?

Hello, I am using the QIAamp Fast DNA Stool Mini Kit for DNA isolation from mouse fecal samples. My results are showing both low DNA concentration and a low A260/230 ratio. This seems unusual,...

last month 7,873 2 View

Feasibility of sequencing 16S V3–V4 amplicons (Illumina overhang primers) on iSeq 100 (2×150)?

Hello everyone — I have locus-specific V3–V4 primers with Illumina overhangs (sequences below) and access to an iSeq 100 (2×150 bp). Before I proceed, I’d appreciate short practical advice: Is it...

last month 2,333 2 View

T Cell Isolation for RT-PCR?

I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...

13 October 2020 3,786 6 View

How can I analyze my PCR data without control sample?

Hi everyone. In my experiment, I compared different passages (p2, p4 and p6) in one cell line in terms of the same genes and used GAPDH as a housekeeping gene. I have no control, I just compared,...

09 May 2020 5,516 0 View

Does human AB serum use for collagenase inhibition?

Doing SVF from adipose tissue i'm using collagenase NB4 (serva). For inhibition collagenase i want use human ab serum (seracare). Does it work?

02 October 2019 9,426 0 View