T Cell Isolation for RT-PCR?

I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...

12 October 2020 3,583 6 View

What is the formula for heat exchange between mass oil in a tank and ambient?

I have a tank of lube oil which is not insulated and top of tank is a compartment, in other words, tank is the bottom of the auxiliary compartment. The volume of tank is 25.000 liters. Because we...

27 March 2018 1,388 5 View

Difference Between FEA Result and Hand Calculation in Beam Bending?

Hi all, I was trying to model deflection in a rectangular cross-section beam fixed at both ends. There is a Pressure Load acting on the beam (uniformly distributed along the beam). In such...

25 November 2017 3,094 18 View

Could you help me anyone to choose the best configuration of the microscope for mono or dFISH staining of fish chromosomes?

a) Leica DM4B b) Zeiss Axioimager 2 c) Nicon Eclipse NiE d) Olympus BX53 How config is the best performance for FISH staining of chromosome? Could you give me a detailed config of fluorescence...

09 June 2017 1,808 3 View

What is the bottom limit for Stoke's Drag assumption in terms of particle size?

Hello everyone, I was trying to model concentration gradients in a flow-free diffusion chamber using COMSOL. I wanted see what would be the effect of gravity on the concentration gradients that I...

06 June 2017 2,759 3 View

Zwick tensile tester?

25 August 2016 7,316 3 View

Do you know the main active substance in lavandula stoechas?

08 April 2015 9,098 7 View

Is anyone familiar with PHA stimulation of PBMC ?

I will do cell culture with specific patients PBMC. I isolated the PBMC and now will stimulate them with PHA as I want to see Th17 association with the disease. After stimulation I will take the...

26 December 2014 2,879 9 View

Can errors in input data be included in the training phase of ANN?

Let one of the input data is 1.34 (+-0.25 error). Can someone include this error (+-0.25) to input data during training of the ANN?

15 February 2013 9,972 8 View

How to calculate and interpret the odds ratio between genotype and disease?

I have attached the data of my results but I'm not sure how to proceed, could you help me?

11 July 2012 6,000 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

NVT or NPT is preferable for gas adsorption simulation ?

Hi there, In order to perform molecule dynamic simulation on gas adsorption on substrate materials, NVT or NPT ensemble is preferable ? Thanks!

08 February 2021 173 1 View

How can I convert permeability unit from barrer to m^2 for using COMSOL MultiPhysics?

I am looking for gas permeability of PDMS for simulation in COMSOL MultiPhysics and using creeping flow , fluid and matrix properties. The permeability unit in COMSOL is m^2 whereas I have the...

08 February 2021 4,305 2 View