Hi everyone.

In my experiment, I compared different passages (p2, p4 and p6) in one cell line in terms of the same genes and used GAPDH as a housekeeping gene. I have no control, I just compared, for example, I compared the Oct4 gene for these three different passages (likewise gapdh). How can I measure ∆Ct without control sample? Or should I measure ∆∆Ct? (Because if i'm not wrong normalization with housekeeping genes only in ∆∆Ct method)

For an example;

P2; oct4 ct:28,9 / gapdh ct: 23,5

P4; oct4 ct:27,2 / gapdh ct:20,5

P6; oct4 ct:28,9 / gapdh ct:21,1