Hello everyone,

We are currently working on cardiomyocyte isolation. We follow the protocol from the previous study and their lysis buffer consisting of 0.5 mg/ml Liberase TL, DNase1 (Worthington, LK003172), and 10 mM HEPES, dissolved in 1.305 ml DMEM (Dulbecco’s Modified Eagle Medium, high glucose, GlutaMAX Supplement, pyruvate. I am curious if DMEM will suppress Liberase activity by any chance, which makes the lysis buffer not strong enough to lyse the cell and isolate the nuclei.

Thank you so much for your help!