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Questions related from Nguyen Nguyen
Hi everyone! We isolated and sequenced our nuclei, and performed hashing prior to sequencing by combining four samples in a single batch, with each sample being stained with a different TotalSeq...
04 May 2023 5,764 1 View
Hello everyone, We're working on the cardiomyocyte nuclei isolation from the snap-frozen heart, and we are still facing some problems when it comes to determining if they're good/bad nuclei under...
08 December 2022 7,650 2 View
Hello everyone, We are planning to use this protocol for our cardiomyocyte isolation: https://www.jove.com/pdf-materials/4205/isolation-of-cardiomyocyte-nuclei-from-postmortem-tissue I am just...
07 December 2022 3,149 2 View
Hello everyone, After the lysis step of cardiomyocyte isolation, we observed a lot of white dots (attached pic/circled) in the suspension when checking under a bright-field microscope. We wonder...
21 November 2022 1,170 1 View
Hello everyone, We are currently working on cardiomyocyte isolation. We follow the protocol from the previous study and their lysis buffer consisting of 0.5 mg/ml Liberase TL, DNase1 (Worthington,...
11 November 2022 3,850 2 View
I've read an article about quantifying collagen/fibers decomposition in blood vessels, especially in the media/intima of veins and they used angle orientation/anisotropy index. I want to ask which...
26 February 2022 7,299 0 View
25 February 2022 8,424 0 View
