In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

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16 January 2021 8,334 5 View

Can chloroform be effectively stabilized with isopropanol? And why does ethanol stabilization usually only involve 1% by mass?

The oxidation of chloroform is dangerous because it results in phosgene formation under exposure to UV light. Along with the minimization of UV exposure, this reaction is usually prevented with 1%...

30 December 2020 3,356 3 View

How can i describe the different bands on tlc plate, plant extract?

On tlc plate i get some colorful band under uv 360nm, like pink, brown, indigo, sky blue and some others. How can i tell that this color indicates this compound? And in which extract hexane,...

21 December 2020 3,423 3 View

What glass vials do you use for small volumes (400ul-2000ul) that fit in the centrifuge?

I am planning some NMR experiments that require glass vials (due to chloroform). I am looking to find glass vials that would take 400-2000ul volumes, fit in the centrifuge and allow me to separate...

01 December 2020 3,259 2 View

Why does the command ''Topshim'' fails ?

Hello, I'am trying to run the 1H NMR spectrum of an ionic solution (Bromide Salt). the solution is too viscous so I had to randomly add a certain volume of deuterated chloroform, and then...

30 November 2020 8,569 1 View