I have isolated Botryococcus in a river, when I analysed it through LM it has colonies and mucilage threads connecting. But when I tried culturing it became unicellular but there are some visible threads, but it has a thick cup shape mucilage on the outer cell and takes 14 days to culture while optimising its culture conditions. But later on ill do some ecological studies and phylogenetic studies on that river. But my theory is that the botryococus I have isolated is not in river itself but the source of it at top or upstream. I hope you can help me.
Lawrence:
This link may prove helpful for your studies:
http://www.globalsciencebooks.info/Online/GSBOnline/images/2010/GGG_4(SI1)/GGG_4(SI1)65-69o.pdf
Best
Syed
Make sure you have a pure culture. The unicellular could be anything.
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