Do they work well?
If you know any articles, I will be very grateful.
15 May 2024 6,791 4 View
See question. Don't know if it's just me but the prices I am seeing for tricaine are high and the paper is just a college paper, sort of.
09 October 2022 6,583 1 View
Good day everybody. I am looking for a good advice what stereomicroscope is capable to detect GFP fluorescent through the Petri dish. My objects are calli, plantlets, etc in vitro, so i want them...
24 November 2021 3,022 3 View
I'm searching for aromatic substances which can be applyed with an olfaktometer in a VR scenario. For this, the aromatic substances must not be harmful to health. They should have a scent of "fire...
19 December 2018 8,438 3 View
I am not a specialist in genome annotation procedures. I found a good review "A beginner's guide to eukariotic genome annotation" written by M.Yandell and D. Ence in 2012. They mention that...
10 October 2018 433 4 View
To be short, I would like to share some thoughts about my research topic, but in common words and without details. Otherwise, I would write a scientific review for that purpose. I am sorry for...
16 October 2017 9,677 3 View
I already have the english version of MOBID-2 Pain scale, but I am looking for a validated german version. Thanks
30 January 2017 2,459 2 View
I am blotting for a human truncated STAT3 (no N-terminus, no C-terminus). Looking through the biggest antibody supplier’s homepages, I only found antibodies specific for either the N or C-terminus...
15 December 2016 661 3 View
I am looking for rough recommended concentrations but I have only found in vitro treatment concentrations.
01 December 2016 6,471 1 View
Currently I am working on palliative sedation of dying patients with symptoms of existential suffering and so I am searching for appropriate assessment-tools for these patients especially when...
15 May 2016 6,987 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
